Robinso, Corey
College of Science and Mathematics
Professor: Dr. Susan Cook
ABSTRACT:
As the field of cellular reprogramming has been an expanding focus of research, a culture method for generating varieties of cell types is a necessity. Hematopoietic stem cells (HSCs) are one cell type that can be induced to differentiate into the formed elements of the blood. Hence, I removed such cells from the bone marrow of out-bred mice and cultured them in RPMI with 10% bovine serum for 7 days. This allowed stromal feeder cells to proliferate. Such cells donate necessary factors for the proliferation of stem cells. After 7 days in RPMI-1640 with 10% bovine serum granulocyte colony stimulating factor (G-CSF) was added at a concentration of 4 uL. Subsequently, granulocytes were seen on culture day 21 among many undifferentiated HSCs. Increasing G-CSF concentration would likely result in higher yield of granulocytes, indicating an effective culture method to generate various lines of blood cells from HSCs. In the future, it may be possible to generate other tissues from hematopoietic stem cells.
